Many genome sequences in public databases are used in metagenomic studies to understand the human microbiome. Unfortunately, many of the taxonomic identities of these genomes are either incorrect or incomplete. Since bacterial species are now defined by comparing genome sequences, having the genome sequence should lead us to the correct identification of any organism at the species, or even subspecies level. The only prerequisite condition is the availability of relevant type strain genomes. In this series of blogs, we will present the correct taxonomic identification of some misidentified genomes frequently used in the publications. These genome sequences were identified using our TrueBac IDTM service.
This strain likely belongs to the species that are widely found in the human gut microbiome as it has appeared in many publications.
In NCBI and other databases, it is labeled as “Ruminococcus sp.” under the genus Ruminococcus and the family Ruminococcaceae.
With the TrueBac ID’s algorithm, this genome was identified as a strain of Blautia wexlerae in the genus Blautia and the family Lachnospiraceae by average nucleotide identity (ANI) value of 98.05% to the type strain of Blautia wexlerae. ANI values of 95~96% are considered as the cut-off for the species boundary [Learn more]. Clearly, it is not a member of Ruminococcus. To browse this genome at EzBioCloud database, visit here. Below is a screen-shot of the TrueBac ID result showing what is now classified as Blautia wexlerae 5_1_39BFAA.
Sept. 17, 2018
Jon is a scientist & entrepreneur dedicated to developing bioinformatics related to bacterial systematics, genomics, and microbiome. He is a professor at Seoul National Univ. and founder of ChunLab, Inc. He is best known as a creator of EzBioCloud (formerly EzTaxon) database, and recipient of Bergey Award.