Bacterial identification is the process of assigning a strain to a known species that was previously classified and named using modern taxonomical principle. In theory, the accuracy of identification is guaranteed if it uses the same method as classification process. At present, bacterial classification is based on the comparison of whole genome sequences, as a form of pairwise genome similarity called Average Nucleotide identity (ANI) [Leran more]. Because TrueBac ID uses the taxonomically verified reference database and scientifically proven algorithm, the results of identification are more accurate than any other technologies. If the genome sequence of an isolate shows >96% ANI value to the taxonomically verified genome sequence of the type strain of Vibrio cholerae,  it is identified as Vibrio cholerae with 100% accuracy, scientifically.


How accurate is the TrueBac ID’s identification?

Bacterial identification is the process of assigning a strain to […]

Limit of raw NGS data

For BIOiPLUG Microbiome Taxonomic Profiling cloud service, up-to 100,000 reads […]

Can I use reverse-complement 16S sequences for “Identify” service?

Yes. EzBioCloud’s “Identify” engine automatically recognizes reverse-complement sequences. They are […]

Why does EzBioCloud yield different similarity values compared to BLAST or other services?

Pairwise sequence similarity is the key criterion for microbial identification […]

Some of my identification results are lost. Where are they?

For public users, up to 200 results are saved. If […]

Is it possible to identify multiple 16S rRNA sequences at once?

Up to ten identifications can be running simultaneously, but they […]

How regularly do you update your EzBioCloud database?

We plan to update our database every two months, starting […]