Frequently asked questions
Yes. EzBioCloud’s “Identify” engine automatically recognizes reverse-complement sequences. They are corrected before being fed to our identification pipeline. We save the corrected sequences in your account, instead of the original version.
Pairwise sequence similarity is the key criterion for microbial identification and as suggested by Kim et al. (2014), a species boundary is defined by a similarity value of 98.65% or greater. In other words, if two sequences show a similarity of 98.65% or less, they belong to different species. BLAST and other services may produce different similarities as they use different algorithms. However, we recommend avoiding BLAST for taxonomy-related sequence identity values. Learn more
For public users, up to 200 results are saved. If a new result is added, the oldest result will be deleted.
Up to ten identifications can be running simultaneously, but they need to be submitted one by one. The ‘Identify single sequence’ button allows the user to submit a single 16S rRNA gene sequence for identification, but the user can sequentially submit additional single sequences for identification, and the system will allow up to 10 identification jobs running simultaneously per user.
We plan to update our database every two months, starting in January 2017.
Bacterial identification is the process of assigning a strain to a known species that was previously classified and named using modern taxonomical principle. In theory, the accuracy of identification is guaranteed if it uses the same method as classification process. At present, bacterial classification is based on the comparison of whole genome sequences, as a form of pairwise genome similarity called Average Nucleotide identity (ANI) [Leran more]. Because TrueBac ID uses the taxonomically verified reference database and scientifically proven algorithm, the results of identification are more accurate than any other technologies. If the genome sequence of an isolate shows >96% ANI value to the taxonomically verified genome sequence of the type strain of Vibrio cholerae, it is identified as Vibrio cholerae with 100% accuracy, scientifically.
BIOiPLUG Microbiome Taxonomic Profiling (MTP)
For BIOiPLUG Microbiome Taxonomic Profiling cloud service, up-to 100,000 reads can be processed per each sample. This limit is after QC and merging paired-end reads and only applied to our free trial, BASIC and PREMIUM subscription models. However, our ENTERPRISE model has no limit on the size of raw data [Learn more].
The following demonstration microbiome contains >9 million reads of Illumina MiSeq V3V5 sequencing which was processed by BIOiPLUG MTP pipeline.