Powered by Precision,
Driven by Quality

OrthoANI genomic similarity

Subscribe To Our Newsletter

Get updates and learn from the best

OrthoANI (Orthologous Average Nucleotide Identity) is a kind of similarity values between two genome sequences. It is an improved version of the original ANI (Average Nucleotide Identity) and is one of the OGRIs. It can be used for classification and identification of Bacteria, and the proposed cutoff for species boundary is 95~96%. The algorithm has been published by Lee et al. (2015) and the software tools are available here . Later, we developed a faster version, named OrthiANIu, using usearch program instead of BLAST. The software tools are available as web-service and standalone program.

  • To calculate OrthoANIu between two genomes, visit here.
  • To download standalone program, visit here.

OrthoANIu is the standard algorithm used to build the EzBioCloud database. The publication for OrthoANIu tool is available here.

The major differences between the original ANI and OrthoANI are:

  • For the original ANI, you need to obtain the reciprocal values (i.e., A->B & B->A), and use the mean value for taxonomic use. In contrast, you only need a single value (A<->B) for OrthoANI.
  • OrthoANI is faster than the original ANI.

Last updated on Sep 11, 2017

Subscribe To Our Newsletter

Get updates and learn from the best

More To Explore

Plasmid and megaplasmid

A plasmid is a DNA molecule that is not chromosomal or extra-chromosomal. It is found in Bacteria and Archaea, and not essential to survival. A

Gene frequency plot in pan-genome

All potential orthologous protein-coding  genes (=CDSs) are clustered into non-redundant gene sets after pan-genome calculation to generate “Pan-genome Orthologous Groups (POGs)”. Obviously, a core part

Share This Post

Share on facebook
Share on linkedin
Share on twitter
Share on email
small_c_popup.png

Have a Question? Let's have a chat?

We're here to answer any question you might have

small_c_popup.png

Have a Question? Let's have a chat?

We're here to answer any question you might have

small_c_popup.png

Stay up to date

Keep up with our latest developments