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[Quiz] Comparing BLAST and EzBioCloud search

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Objectives:

Understanding the DNA sequence searching algorithm and difference from the sequence similarity calculation.

Please do the followings:

  1. Select randomly five 16S sequences from this data set.
  2. Search them against NCBI NR/NT database at https://blast.ncbi.nlm.nih.gov/Blast.cgi.
  3. Search them using EzBioCloud “Identify” at https://www.ezbiocloud.net/identify.

Please answer the following questions:

  1. Summarize the results of two searches.
  2. Are the top hits are the same? If not, why?
  3. What are the meanings of scores/indexes given by BLAST and EzBioCloud searches?
  4. Is there similarity/identity measure in two searches? Are they the same or not? Can they be used for the taxonomic purposes?

By Jon Jongsik Chun

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