Powered by Precision, Driven by Quality

[Quiz] Comparing BLAST and EzBioCloud search

Subscribe To Our Newsletter

Get updates and learn from the best

Objectives:

Understanding the DNA sequence searching algorithm and difference from the sequence similarity calculation.

Please do the followings:

  1. Select randomly five 16S sequences from this data set.
  2. Search them against NCBI NR/NT database at https://blast.ncbi.nlm.nih.gov/Blast.cgi.
  3. Search them using EzBioCloud “Identify” at https://www.ezbiocloud.net/identify.

Please answer the following questions:

  1. Summarize the results of two searches.
  2. Are the top hits are the same? If not, why?
  3. What are the meanings of scores/indexes given by BLAST and EzBioCloud searches?
  4. Is there similarity/identity measure in two searches? Are they the same or not? Can they be used for the taxonomic purposes?

By Jon Jongsik Chun

Subscribe To Our Newsletter

Get updates and learn from the best

More To Explore

TrueBac ID – How to upload your genome

TrueBacTM ID upload center currently supports two types of sequence files, namely, FASTA, and FASTQ file(s) (single and paired-end). All these files can be uploaded

16S rRNA and 16S rRNA Gene

Overview 16S rRNA stands for 16S ribosomal ribonucleic acid (rRNA), where S (Svedberg) is a unit of measurement (sedimentation rate). This rRNA is an important

Share This Post

Share on facebook
Share on linkedin
Share on twitter
Share on email
small_c_popup.png

Have a Question? Let's have a chat?

We're here to answer any question you might have

small_c_popup.png

Have a Question? Let's have a chat?

We're here to answer any question you might have

small_c_popup.png

Stay up to date

Keep up with our latest developments