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[Quiz] Comparing BLAST and EzBioCloud search

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Objectives:

Understanding the DNA sequence searching algorithm and difference from the sequence similarity calculation.

Please do the followings:

  1. Select randomly five 16S sequences from this data set.
  2. Search them against NCBI NR/NT database at https://blast.ncbi.nlm.nih.gov/Blast.cgi.
  3. Search them using EzBioCloud “Identify” at https://www.ezbiocloud.net/identify.

Please answer the following questions:

  1. Summarize the results of two searches.
  2. Are the top hits are the same? If not, why?
  3. What are the meanings of scores/indexes given by BLAST and EzBioCloud searches?
  4. Is there similarity/identity measure in two searches? Are they the same or not? Can they be used for the taxonomic purposes?

By Jon Jongsik Chun

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More To Explore

Microbiomes revealed by taxonomic profiling

The following data were processed by ChunLab’s 16S Microbiome Profiling Service with EzBioCloud database. You can access the taxonomic profiles without login.   Sample NGS

About EzBioCloud.net

EzBioCloud is ChunLab’s data and analytics portal focusing on taxonomy, ecology, genomics, metagenomics, and microbiome of Bacteria and Archaea. The service runs on the Amazon

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