TrueBacTM ID upload center currently supports two types of sequence files, namely, FASTA, and FASTQ file(s) (single and paired-end). All these files can be uploaded in gzipped, zipped, and unzipped format.
The very first step in uploading process involves selection of upload platforms. This will be vary depending on the file(s) you wish to upload.
The following extension lists are commonly used names for given formats.
- FASTA: ‘*.fasta’, ‘*.fna’, ‘*.fna_nt’, ‘*.fas’, ‘*.fsa’, ‘*.fa’
- FASTQ: *.fastq, *.fq
After the platform selection, you can simply drag and drop the file(s) to the web browser or click a “Select FASTA/Q files” button to locate the file(s).
*Please note that for paired-end FASTQ files, they need to have the following naming convention for automatic paired-end detection.
- SampleName_SampleNumber_Lane_Read_FlowCellIndex.fastq.gz
- e.g. SampleName_S1_L001_R1_001.fastq.gz
- SampleName_S1_L001_R2_001.fastq.gz
If provided correctly the upload center will pair up the files into a single sample. If not you may have to pair up the files manually.
Once the data has successfully uploaded, click “Request pipeline run” button to start the TrueBacTM ID analysis. “Run Status” on the side will show the current status of your ID process.
Last updated Feb 6, 2019
