Powered by Precision, Driven by Quality

[Tutorial] 16S microbiome analysis of ATCC(R) Microbiome Standards

Subscribe To Our Newsletter

Get updates and learn from the best

Several standards products are available to validate 16S microbiome sequencing procedures. They are usually a mixture of genomic DNAs or whole cells from different species whose composition is precisely known. At present, EzBioCloud can be used along with these products including one sold by the American Type Culture Collection (ATCC). In this tutorial, we offer a step-by-step guide to help you use this product.

What to prepare in advance

You need NGS data to upload:

You need NGS data file(s) from 16S amplicon sequencing from ATCC Microbiome Standards product. If it is from Illumina (paired-end or single-end), it should be in FASTQ format. For Pacific Biosciences, it should be in FASTA format (after ccs processing). If you don’t have data, don’t worry. We provide an example file you can download from here .  These ATCC Microbiome Standards were sequenced by ChunLab.

You need an account at EzBioCloud:

You will also need an account at www.EzBioCloud.com. If you don’t have one already, please register at //www.ezbiocloud.net/signup. It is free but requires you use an institutional email account (not gmail etc.). Once you are logged into EzBioCloud.com, you will also need to sign up for a free trial of the Microbiome Taxonomic Profiling (MTP) service. Please visit here to request your free trial.  Your free trial account will let you analyze and compare up to 20 microbiome samples, each containing up to 100,000 reads.

Once you have a EzBioCloud account with a free trial for the MTP service activated, and data to upload, you are ready to go.

What to do in this tutorial

We will do the following in this tutorial. If you want to know further about any specific items, please search within help.ezbiocloud.net.

Please follow the below tutorial. If you have any question, please contact us at info@chunlab.com.


The EzBioCloud team / Last edited on May 22, 2018

Subscribe To Our Newsletter

Get updates and learn from the best

More To Explore

Genome-based Identification for Improving Reference Databases

Misidentified or incompletely identified bacterial genome sequences appear frequently in public reference databases. These databases can be significantly improved by genome-based identification against an up-to-date, systematically curated

Share This Post

Share on facebook
Share on linkedin
Share on twitter
Share on email
small_c_popup.png

Have a Question? Let's have a chat?

We're here to answer any question you might have

small_c_popup.png

Have a Question? Let's have a chat?

We're here to answer any question you might have

small_c_popup.png

Stay up to date

Keep up with our latest developments